HiPrep™ Heparin 16/10 FF Column

Technical Information

TECHNICAL SPECIFICATIONS
Ligand	Porcine heparin
Ligand density	» 5 mg/ml medium
Ligand coupling method	Reductive amination
Matrix	Highly cross-linked agarose, 6%
Average particle size	90 μm
pH stability	4-13 (short term), 4-12 (long term and working range)
Prepacked HiPrep™ 16/10 Columns
Column volume	20 ml
Column dimensions	1.6 × 10 cm
Recommended flow rate*	2-10 ml/min (60-300 cm/h)
Max. flow rate*	10 ml/min (300 cm/h)
Max. pressure over the packed bed during operation*	1.5 bar (22 psi, 0.15 MPa)
Column hardware
pressure limit	5 bar (73 psi, 0.5 MPa)
Storage	0.05 M sodium acetate containing 20% ethanol
Storage temperature	4°C to 30°C
* H₂O at 25°C

HiPrep™ Heparin 16/10 FF for purification of e.g. coagulation factors and DNA binding proteins.