PLK1 monoclonal antibody (M01), clone 2G12
产品名称： PLK1 monoclonal antibody (M01), clone 2G12
英文名称： PLK1 monoclonal antibody (M01), clone 2G12
产品编号： H00005347-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant PLK1.
- Immunogen:
- PLK1 (AAH02369, 1 a.a. ~ 603 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (91.85 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- PLK1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of PLK1 expression in Hela NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Cell lysate)
- Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.
Lane 1: PLK1 transfected lysate(68.3 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunofluorescence
- enlarge
- Immunoprecipitation
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- In situ Proximity Ligation Assay (Cell)
- enlarge
- Entrez GeneID:
- 5347
- GeneBank Accession#:
- BC002369
- Protein Accession#:
- AAH02369
- Gene Name:
- PLK1
- Gene Alias:
- PLK,STPK13
- Gene Description:
- polo-like kinase 1 (Drosophila)
- Omim ID:
- 602098
- Gene Ontology:
- Hyperlink
- Other Designations:
- cell cycle regulated protein kinase,polo (Drosophia)-like kinase,polo like kinase,polo-like kinase
- Interactome
- Gene Pathway